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DNA Structure-Specific Cleavage of DNA-Protein Crosslinks by the SPRTN Protease

Molecular Cell paper from the Stingele lab


Hannah K Reinking, Hyun-Seo Kang, Maximilian J Götz, Hao-Yi Li, Anja Kieser, Shubo Zhao, Aleida C Acampora, Pedro Weickert, Evelyn Fessler, Lucas T Jae, Michael Sattler, Julian Stingele (2020) Mol Cell 80(1): 102-113. doi: 10.1016/j.molcel.2020.08.003

Abstract cited directly from the paper:
Repair of covalent DNA-protein crosslinks (DPCs) by DNA-dependent proteases has emerged as an essential genome maintenance mechanism required for cellular viability and tumor suppression. However, how proteolysis is restricted to the crosslinked protein while leaving surrounding chromatin proteins unharmed has remained unknown. Using defined DPC model substrates, we show that the DPC protease SPRTN displays strict DNA structure-specific activity. Strikingly, SPRTN cleaves DPCs at or in direct proximity to disruptions within double-stranded DNA. In contrast, proteins crosslinked to intact double- or single-stranded DNA are not cleaved by SPRTN. NMR spectroscopy data suggest that specificity is not merely affinity-driven but achieved through a flexible bipartite strategy based on two DNA binding interfaces recognizing distinct structural features. This couples DNA context to activation of the enzyme, tightly confining SPRTN’s action to biologically relevant scenarios.

reinking et al 2020