Unscheduled DNA replication in G1 causes genome instability and damage signatures indicative of replication collisions
Karl-Uwe Reusswig, Julia Bittmann, Martina Peritore, Mathilde Courtes, Benjamin Pardo, Michael Wierer, Matthias Mann & Boris Pfander (18 Nov 2022) Unscheduled DNA replication in G1 causes genome instability and damage signatures indicative of replication collisions. Nature Communications 13 Article number 7014. DOI https://doi.org/10.1038/s41467-022-34379-2
Abstract cited directly from the article:
DNA replicates once per cell cycle. Interfering with the regulation of DNA replication initiation generates genome instability through over-replication and has been linked to early stages of cancer development. Here, we engineer genetic systems in budding yeast to induce unscheduled replication in a G1-like cell cycle state. Unscheduled G1 replication initiates at canonical S-phase origins. We quantifiy the composition of replisomes in G1- and S-phase and identified firing factors, polymerase α, and histone supply as factors that limit replication outside S-phase. G1 replication per se does not trigger cellular checkpoints. Subsequent replication during S-phase, however, results in over-replication and leads to chromosome breaks and chromosome-wide, strand-biased occurrence of RPA-bound single-stranded DNA, indicating head-to-tail replication collisions as a key mechanism generating genome instability upon G1 replication. Low-level, sporadic induction of G1 replication induces an identical response, indicating findings from synthetic systems are applicable to naturally occurring scenarios of unscheduled replication initiation.