Dr. Catherine Regnard

PhD postion available. Job description (pdf, 1.8 MB)

Stability of the coding genome and signaling within the active genome

We have shown that the essential chromosomal kinase JIL-1 forms a stable epigenetic “reader-writer” module with JASPer, which targets the complex to transcribed genes (Albig et al., 2019). The Complex is however not essential for transcription. Our current model is that distribution of the kinase complex genome-wide is important for genome stability at various levels: maintenance of telomers, balance between euchromatin and constitutive heterochromatin and stability of the coding genome in particular. The project aims to elucidate, which specific (stress) signals activate JIL-1 kinase and what are the substrates of JIL-1 besides histone H3. We will concentrate on endogenous sources of DNA damage linked to transcription (e.g. R-loop formation). The JIL-1-dependent phospho-proteome hits will be considered (1) for their potential role in genome stability, (2) for their (conditional) association to the complex interaction network of JIL-1 and/or JASPer, (3) their genome-wide distribution.

Albig C, Wang C, Dann GP, Wojcik F, Schauer T, Krause S, Maenner S, Cai W, Li Y, Girton J, Muir TW, Johansen J, Johansen KM, Becker PB, Regnard C. Nat. Commun. 2019 Nov 25;10(1):5343. doi: 10.1038/s41467-019-13174-6.

*SLiM: Small Linear conserved Motif (FxGF) bound by the LEDGF domain of JASPer. KD: Kinase domain, KDII activates KDI, which then phosphorylates substrates. PEST motifs are found in unstable proteins: JIL-1 is degraded in the absence of JASPer. PWWP domain of JASPer is a reader domain binding nucleosomes with histone H3K36me3

Curriculum Vitae

University degree

• 1988-1994 Biochemistry Diploma “Biology integrative des proteins", University Pierre et Marie Curie, Paris

Advanced academic education

• 1999 Doctorate in Biochemistry "Enzymes polyglutamylating microtubules in neuronal cells", University Pierre et Marie Curie, Paris – Collège de France – Prof. Philippe Denoulet - Laboratoire de biologie cellulaire - “Felicitations du Jury”

Positions held

• Since 2011 Akademische Rätin, LMU, Munich
• 2002-2011 Postdoctoral fellow with Prof. P.B. Becker, “JIL-1 kinase and dosage compensation in flies”, LMU, Munich
• 2000-2002 Postdoctoral fellow with Prof. E. A. Nigg, “Study of the human Cdc14A/B phosphatases”, Max-Planck-Institut für Biochemistry, Martinsried
• 1995-1998 “Monitorat” teaching in Biochemistry, University Pierre et Marie Curie, Paris

Fellowships

• FEBS long term fellowship (two years)
• Association pour la recherche sur le cancer (one year)
• Fondation pour la recherche médicale (six months)
• Bourse de thèse de doctorat - "Major de promotion" (four years)

Reviewing activities

• EMBO Journal
• EMBO reports
• PLOS genetics
• PLOS one

CRC project-related publications

JASPer controls interphase histone H3 Ser10 phosphorylation by chromosomal kinase JIL-1 in Drosophila. Albig C, Wang C, Dann GP, Wojcik F, Schauer T, Krause S, Maenner S, Cai W, Li Y, Girton J, Muir TW, Johansen J, Johansen KM, Becker PB and Regnard C. Nat Commun. 2019 Nov 25;10(1):5343. doi: 10.1038/s41467-019-13174-6.

Kinase -mediated changes in nucleosome conformation trigger chromatin decondensation via poly(ADP-ribosyl)lation.Thomas CJ, Kotova E, Andrake M, Adolf-Bryfogle J, Glaser R, Regnard C, Tulin AV. Mol Cell. 2014 Mar 6;53(5):831-42. doi: 10.1016/j.molcel.2014.01.005. Epub 2014 Feb 6.

Global analysis of the relationship between JIL-1 kinase and transcription. Regnard C, Straub T, Mitterweger A, Dahlsveen IK, Fabian V, Becker PB. PLoS Genet. 2011 Mar;7(3):e1001327. doi: 10.1371/journal.pgen.1001327. Epub 2011 Mar 10.

Phosphorylation of SU(VAR)3-9 by the chromosomal kinase JIL-1. Boeke J, Regnard C, Cai W, Johansen J, Johansen KM, Becker PB, Imhof A. PLoS One. 2010 Apr 6;5(4):e10042. doi: 10.1371/journal.pone.0010042.