qPCR Seminar
Best practices for quantitative PCR
19.02.2020
Registration is open!
Date and Time: February 19, 2020, from 13:15-16:00
Location: BMC, room N02.017
Trainer: Dr. Jan Winter, Application Specialist at Steinbrenner Lab Systems
Dr. Jan Winter will offer sessions for both beginners and experienced users: qPCR basics, followed by an advanced session with troubleshooting tips. Participants can state their own qPCR questions and/or challenges on the registration sheet.
Open to users of all levels – students, doctoral researchers, postdocs, technicians.
Participation is free of charge. Space is limited –registration required! https://seminar.qpcr-guide.com
Download invitation/description (pdf, 3 MB)
Best practices for quantitative PCR, program
13:15-14:15 Session 1: qPCR Basics and Optimal Assay Design
- Background Knowledge
- Applications
- ASsay Design
- TaqMan or Dye?
- Primer Design
- Multiplexing
- Assay Validation
Coffee break
14:45-15:45 Session 2: Advanced Topics and Troubleshooting
- Data Analysis and Software
- Pitfalls
- Common mistakes
- Strategies for Assay Optimization
- Troubleshooting of your specific problems