A22 - Functional interplay between DNA methylation and polycomb during preimplantation development
At present, the relationship between DNA methylation and Polycomb is poorly understood. Our preliminary data indicate an extensive DNA methylation turnover at H3K27me3 marked promoters during epiblast differentiation. We will investigate the establishment of this DNA methylation equilibrium by combining state-of-the-art gene editing and genome-wide sequencing technologies. Furthermore, we will identify the consequences of the DNA methylation turnover for cell fate commitment on a single cell level. This will greatly contribute to our understanding of the intricate interplay between DNA methylation and Polycomb and its function during development and disease.