A01 - Domino nucleosome remodeling complexes and histone H2A.V dynamics in transcription and DNA repair
In Drosophila melanogaster the histone H2A.V variant plays diverse roles as DNA damage sensor and architectural element in the chromatin of active promoters. Accordingly, placement and turnover of the variant through the nucleosome remodeling ATPase Domino becomes a crucial determinant for these functions. The two alternative DOM splice variants have distinct cell type-specific and developmental functions mediated by H2A.V exchange. Applying tools and methods generated during the preceding funding period we plan to determine the subunit composition of Domino remodeling complexes and to explore how they affect H2A.V dynamics in the context of promoter architecture and the DNA damage response.