A32 - Protein and phosphorylation nuclear dynamics in time and space and its modulation by metabolism
In this project we will study how the molecular mechanism of the circadian clock in the nucleus is aided by systemic and/or tissue specific co-regulators and how this is impaired by metabolic disruption. We will employ mass spectrometry (MS)-based quantitative proteomics to characterize and compare daily proteome and phosphoproteome oscillations in soluble and chromatin enriched nuclear fractions in three different organs of mice fed with normal or high fat diet (HFD). In addition, by using chromatin immunoprecipitation with MS we will analyze how HFD rewires the molecular composition of core clock complexes at the chromatin to disrupt circadian tissue homeostasis.
