A06 - Structure and Mechanism of the INO80 complex
PhD position available.
The INO80 complex is 1.2 MDa 20 subunit chromatin remodeler with broad roles in DNA repair, replication and transcription. Recent results show that INO80 positions +1 and -1 nucleosomes at promoters and generates nascent nucleosome arrays. Our goal is to establish a structural framework of INO80 and to reveal how the remodeler positions and slides nucleosomes by combining cryo-electron microscopy, X-ray crystallography, mass spectrometry, and biochemical analyses.
We obtained different pieces of information on the chromatin remodeler INO80 from integrated structural methods. Each puzzle piece brought us one step closer to the 3D model of INO80 and the process of chromatin remodeling.