A21 - Nutritional reprogramming of chromatin interactions by the glucocorticoid receptor
The Glucocorticoid Receptor (GR) is a ligand-gated transcription factor that controls essential physiological processes at the chromatin level. We have identified a putative high fat diet induced GR co-regulator, which is a member of the Inhibitor of Histone Acetylation complex (INHAT), in mouse livers. Our goal is to characterize its functions in genomic responses to nutrition and GR signaling. By ChIP- & RNA-seq, we will profile its genome-wide occupancy, linked histone modifications and transcriptional targets. Its impact on chromatin dynamics will be determined by studying both physiological and molecular consequences of hepatic gain and loss of function mouse models, using viral vectors and nanoparticle-coupled siRNAs in vivo.
Cistromic reprogramming of hepatic chromatin signatures by high fat diet feeding. Representative ChIP-Seq browser tracks of H3K27 acetylation patterns from mouse livers.